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Characterization of BAT‐EVs from Male and Female Mice. (A) Concentration of BAT‐EVs from C57BL/6 male mice showing the greatest BAT‐EV concentration among the tested collagenase conditions at 0.50 mg/mL collagenase <t>type</t> <t>1</t> concentration after incubation for 48 h post 4 h equilibration. (B) Concentration of BAT‐EVs from C57BL/6 female mice showing the highest concentration of BAT‐EVs at 0.50 mg/mL collagenase type 1 concentration after incubation for 48 h post 4 h equilibration. (C) The mean size of BAT‐EVs from male mice was significantly lower with 0.25 mg/mL and 0.50 mg/mL collagenase type 1 concentration. (D) The mean size of BAT‐EVs from female mice was lower with 0.50 mg/mL collagenase concentration. (E) Size distribution of the BAT‐EVs obtained from male mice. (F) show the size distribution of the BAT‐EVs obtained from female mice. N = 3–4 per group; Data are mean ± SEM; * p < 0.05; ** p < 0.01; *** p < 0.001.
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Characterization of BAT‐EVs from Male and Female Mice. (A) Concentration of BAT‐EVs from C57BL/6 male mice showing the greatest BAT‐EV concentration among the tested collagenase conditions at 0.50 mg/mL collagenase <t>type</t> <t>1</t> concentration after incubation for 48 h post 4 h equilibration. (B) Concentration of BAT‐EVs from C57BL/6 female mice showing the highest concentration of BAT‐EVs at 0.50 mg/mL collagenase type 1 concentration after incubation for 48 h post 4 h equilibration. (C) The mean size of BAT‐EVs from male mice was significantly lower with 0.25 mg/mL and 0.50 mg/mL collagenase type 1 concentration. (D) The mean size of BAT‐EVs from female mice was lower with 0.50 mg/mL collagenase concentration. (E) Size distribution of the BAT‐EVs obtained from male mice. (F) show the size distribution of the BAT‐EVs obtained from female mice. N = 3–4 per group; Data are mean ± SEM; * p < 0.05; ** p < 0.01; *** p < 0.001.
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Characterization of BAT‐EVs from Male and Female Mice. (A) Concentration of BAT‐EVs from C57BL/6 male mice showing the greatest BAT‐EV concentration among the tested collagenase conditions at 0.50 mg/mL collagenase type 1 concentration after incubation for 48 h post 4 h equilibration. (B) Concentration of BAT‐EVs from C57BL/6 female mice showing the highest concentration of BAT‐EVs at 0.50 mg/mL collagenase type 1 concentration after incubation for 48 h post 4 h equilibration. (C) The mean size of BAT‐EVs from male mice was significantly lower with 0.25 mg/mL and 0.50 mg/mL collagenase type 1 concentration. (D) The mean size of BAT‐EVs from female mice was lower with 0.50 mg/mL collagenase concentration. (E) Size distribution of the BAT‐EVs obtained from male mice. (F) show the size distribution of the BAT‐EVs obtained from female mice. N = 3–4 per group; Data are mean ± SEM; * p < 0.05; ** p < 0.01; *** p < 0.001.

Journal: The FASEB Journal

Article Title: Isolation of Extracellular Vesicles From Mouse Brown Adipose Tissue Secretome Using Size‐Exclusion Chromatography

doi: 10.1096/fj.202600635R

Figure Lengend Snippet: Characterization of BAT‐EVs from Male and Female Mice. (A) Concentration of BAT‐EVs from C57BL/6 male mice showing the greatest BAT‐EV concentration among the tested collagenase conditions at 0.50 mg/mL collagenase type 1 concentration after incubation for 48 h post 4 h equilibration. (B) Concentration of BAT‐EVs from C57BL/6 female mice showing the highest concentration of BAT‐EVs at 0.50 mg/mL collagenase type 1 concentration after incubation for 48 h post 4 h equilibration. (C) The mean size of BAT‐EVs from male mice was significantly lower with 0.25 mg/mL and 0.50 mg/mL collagenase type 1 concentration. (D) The mean size of BAT‐EVs from female mice was lower with 0.50 mg/mL collagenase concentration. (E) Size distribution of the BAT‐EVs obtained from male mice. (F) show the size distribution of the BAT‐EVs obtained from female mice. N = 3–4 per group; Data are mean ± SEM; * p < 0.05; ** p < 0.01; *** p < 0.001.

Article Snippet: Chemical reagents: Medium 199/EBSS (Cytiva, SH30253.01) Penicillin–streptomycin (Invitrogen, 15140‐155) Insulin (Roche, 11376497001) Dexamethasone (Sigma, D‐4902) Collagenase type 1 (Worthington Biochemical Corporation, LS004197 ) Sterile Phosphate buffered saline (PBS) Sterile ultrapure distilled water (UPDW) Sterile filtered 0.5 M NaOH 70% Ethanol 20% Ethanol Supplies and Equipment: Dissection tools: straight iris scissors and straight forceps 3 × 3 Gauze 12‐well cell culture plate, non‐treated (VWR, 10861‐698) 0.2 μM Vacuum Filter Unit (VWR, 10040‐436) Sterile Serological pipet tips‐25mL Sterile Serological pipet tips‐10mL Single channel pipetters (20 μL, 200 μL, 1000 μL) Polypropylene micro tubes‐1.5 mL qEV 35 nm Columns from Izon with 1 mL loading volume (Izon, IC1‐35) Funnel‐shaped reservoir provided with each qEV Column.

Techniques: Concentration Assay, Incubation